Rolling circle–based linear amplification
WebOct 29, 2024 · The RCA-based synthetic route of DNA hydrogel synthesis includes three essential stages (Fig. 1 ): (1) preparation of precursor solution containing circ-DNA as the template for RCA; (2)... WebLau HY, Botella JR (2024) Advanced DNA-based point of care diagnostic methods for plant diseases detection. Plant Sci 8:2016 Lizardi PM, Huang XH, Zhu ZR, Bray-Ward P, Thomas DC, Ward DC (1998) Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nat Genet 19:225–232
Rolling circle–based linear amplification
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WebTo help address this urgent need, we have developed a sensitive method for nucleic acid analysis based on padlock probe rolling circle amplification (PLRCA), nuclease protection … WebJun 24, 2024 · Based on time-cost analysis and comparative activity data, a cell-free workflow using synthetic DNA minicircles and rolling circle amplification enables …
WebJan 1, 2006 · Rolling circle amplification (RCA) of plasmid or genomic DNA using random hexamers and bacteriophage phi29 DNA polymerase has become increasingly popular in the amplification of template DNA in DNA sequencing. WebNov 7, 2012 · These include nucleic acid sequence-based amplification (NASBA), loop-mediated amplification (LAMP), helicase-dependent amplification (HDA), rolling circle …
WebRolling-circle amplification (RCA) products as DNA probes for fluorescent in situhybridization (FISH). (a) Scheme of the RCA process. After thermal denaturation, the random hexamer primers hybridize to the circular DNA template at multiple sites. The amplification starts, extending each primer by the Φ29 DNA polymerase. Webamplifies amultimeric cDNA by rolling-circle mechanism but only amonomeric cDNA with linearRNA template by normal amplification, if first-generation primers (red) are used.The …
WebAug 28, 2024 · Rolling circle amplification (RCA) is a robust enzymatic process in which a circular DNA molecule serves as a template for exponential amplification of molecular …
WebFeb 1, 2024 · In 2024, Li et al. developed an alternative PCR-based assay that avoids the problems of linear RNA interference and rolling circle amplification ( Fig. 2) [ 63 ]. This assay uses a pair of DNA probes designed to complement each side of the backsplice junction site of a target circRNA. bone of the bone and flesh of the fleshWebMay 1, 2011 · This method transfers the exponential amplification profile into a linear, digital format. ... , 23 nucleic acid sequence based amplification (NASBA), 24 … bone of the earWebHerein, we proposed a highly loaded Na +-fueled linear programmable DNAzyme nanostructure (LPDN) composed of long, single-strand DNA produced by rolling circle amplification reactions that served as binding partners for Na +-specific DNAyme and substrate. In the meantime, the long, programmable scaffolds can precisely control the … goat\\u0027s-beard b0WebAug 1, 2024 · Here, we present an isothermal strategy named rolling circle reverse transcription-mediated RNA amplification (RCRT-MRA) to amplify small RNAs with accurate length and sequence. The target RNA and complementary DNA were circularized to serve as amplicons replicated via the rolling circle mechanism. goat\u0027s-beard bWebFeb 13, 2024 · Most natural DNA and RNA are devoid of long trinucleotide (TN) sequences that lack one specific nucleotide (missing nucleotide (MN)). Here we developed a novel … goat\\u0027s-beard b1WebOct 27, 2024 · 1. A method for making a physical map of rolling circle amplification (RCA) products, comprising: (a) producing a complex comprising population of grid oligonucleotide molecules and a population of RCA products that each have a unique RCA product identifier sequence, wherein the grid oligonucleotides are hybridized directly or indirectly via a splint … goat\\u0027s-beard bWebDec 30, 2024 · MicroRNAs (miRNAs) are useful biomarkers for the diagnosis of a variety of cancers. However, it is a major challenge to detect miRNAs, considering their high sequence similarity, low concentration, and small size nature. With the establishment of an efficient rolling circle amplification (RCA) molecular network by target-driven … goat\u0027s-beard b1